Anti-Histone H2A antibody - ChIP Grade ab18255 from Abcam

Product Specs
ItemAnti-Histone H2A antibody - ChIP Grade
CompanyAbcam
Price Pricing Info Supplier Page View Supplier Product Page
Catalog Numberab18255
Antibody TypePrimary
HostRabbit
Quantity100 UG
IsotypeIgG
ApplicationsChIP, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
ClonalityPolyclonal
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaEpigenetics
Gene NameHIST1H2AB
ReactivityCow, Human, Mouse, Recombinant fragment
TargetHistone H2A

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Detection of cell-free histones in the cerebrospinal fluid of pediatric central nervous system malignancies by imaging flow cytometryFrontiers in Molecular BiosciencesNovember 1, 2023Diana Buzova, Jan Frohlich, Danica Zapletalova, Marco Raffaele, Oriana Lo Re, Desislava K. Tsoneva, Jaroslav Sterba, Jan Cerveny, Manlio VinciguerraFisher Scientific, A-21432, United States). Third set. Primary antibodies: anti-histone H2A (Abcam, Ab18255, United States), anti-histone H2B (Abcam, Ab134211, United States), anti-histone H4 (Abcam, Ab31830,

(2) Competitive Chemical Reaction Kinetic Model of Nucleosome Assembly Using the Histone Variant H2A.Z and H2A In VitroInternational Journal of Molecular SciencesOctober 31, 2023Hongyu Zhao, Xueqin Shao, Mingxin Guo, Yongqiang Xing, Jingyan Wang, Liaofu Luo, Lu Caimilk at room temperature in a TBST buffer, and the indicated antibodies (H2A: Abcam, Waltham, MA, USA, ab18255; H2A.Z: Abcam, ab4174; H4: Abcam, ab31830) were used. The immunoreactive bands were visualized using

(3) Single molecule analysis of CENP-A chromatin by high-speed atomic force microscopyeLifeSeptember 20, 2023Daniël P Melters, Keir C Neuman, Reda S Bentahar, Tatini Rakshit, Yamini Dalalpolyclonal),Santa Cruz,Cat. #: sc-22789,WB (1:500) Antibody,Anti-H2A (rabbit polyclonal),Abcam,"Cat. #: ab18255, RRID: AB_470265",WB (1:1000) Antibody,Anti-CENP-A (rabit polyclonal),This paper,,nChIP (3 µL per test) Antibody,"ACA

(4) Multiomics analysis of naturally efficacious lipid nanoparticle coronas reveals high-density lipoprotein is necessary for their functionNature CommunicationsJuly 6, 2023Kai Liu, Ralf Nilsson, Elisa Lázaro-Ibáñez, Hanna Duàn, Tasso Miliotis, Marie Strimfors, Michael Lerche, Ana Rita Salgado Ribeiro, Johan Ulander, Daniel Lindén, et al.with primary antibodies against LDLr (#ab30532, Abcam), SRB1 (#ab217318, Abcam), and/or Histone H2A (#ab18255, Abcam), where appropriate, all diluted 1:2000 in blocking buffer at for overnight at 4 oC. Membranes

(5) Multi-layered chromatin proteomics identifies cell vulnerabilities in DNA repairNucleic acids researchJanuary 25, 2023Sigismondo G, Arseni L, Palacio-Escat N, Hofmann TG, Seiffert M, Krijgsveld J.,Novus Biologicals,NB100-304 Human XRCC6,ThermoFisher Scientific,MA1-21818 Human γH2AX,Merk Millipore,05-636 Human H2A,Abcam,ab18255 Biotin mouse,Sigma-Aldrich,SAB4200680 Biotin rabbit,Cell Signaling Technology,D5A7 Mouse IgG,Santa Cruz Biotechnology

(6) Differential requirement for BRCA1-BARD1 E3 ubiquitin ligase activity in DNA damage repair and meiosis in the Caenorhabditis elegans germ linePLoS geneticsJanuary 1, 2023Li Q, Kaur A, Okada K, McKenney RJ, Engebrecht J.protein; IgG2b mAb; 1:1000; RRID: AB_2532070; in-house (Trimmer Laboratory)], or anti-Histone-H2A (ab18255; Abcam; 1:1000; RRID:AB_470265) followed by IRDye800-conjugated anti-mouse IgG secondary antibodies

(7) Profiling of cell‐free DNA methylation and histone signatures in pediatric NAFLD: A pilot studyHepatology CommunicationsDecember 1, 2022Diana Buzova, Maria Rita Braghini, Salvatore Daniele Bianco, Oriana Lo Re, Marco Raffaele, Jan Frohlich, Antoniya Kisheva, Annalisa Crudele, Antonella Mosca, Maria Rita Sartorelli, et al.Fluor 555 (Ab150130; Abcam). For the third staining set, the primary antibodies were anti‐macroH2A (Ab18255; Abcam), anti‐histone H2B (Ab134211; Abcam), anti‐histone H4 (Ab31830; Abcam), and anti‐histone H3 (Ab12079;

(8) Circ_0004087 interaction with SND1 promotes docetaxel resistance in prostate cancer by boosting the mitosis error correction mechanismJournal of experimental & clinical cancer research : CRJune 3, 2022Chen L, Song Y, Hou T, Li X, Cheng L, Li Y, Xing Y.anti-C-Myb (17,800–1-AP, Proteintech, USA), anti-BUB1 (13,330–1-AP, Proteintech, USA), anti-Histone H2A (ab18255, Abcam, UK), anti-p-Histone H2A (ab177391, Abcam, UK). Plasmids construction and cell transfection Full

(9) Defective repair of topoisomerase I induced chromosomal damage in Huntington’s diseaseCellular and Molecular Life SciencesMarch 1, 2022Nelma M. Palminha, Cleide Dos Santos Souza, Jon Griffin, Chunyan Liao, Laura Ferraiuolo, Sherif F. El-Khamisy(A2052),1:1000,IF DARPP32,Rabbit,Abcam (ab40801),1:100,IF BrdU,Rat,Abcam (ab6326),1:1000,IF H2A,Rabbit,Abcam (ab18255),1:1000,WB RNF168,Mouse,Santa-Cruz Biotechnology (sc-101125),1:1000,WB "Cleaved caspase-3 (Asp175,

(10) Chaperone mediated autophagy contributes to the newly synthesized histones H3 and H4 quality controlNucleic acids researchFebruary 28, 2022Hormazabal J, Saavedra F, Espinoza-Arratia C, Martinez NW, Cruces T, Alfaro IE, Loyola A.(Cell Signaling Technologies, 2891), FLAG (Sigma, F1804), HIRA (Abcam, ab20655), Histone H2A (Abcam, ab18255), Histone H3 (Abcam, ab1791), Histone H4 (Abcam, ab10158), HSC70 (Abcam, 19136), HSP90 (Abcam, ab13492),

(11) Identification of cellular proteins associated with human cytomegalovirus (HCMV) DNA replication suggests novel cellular and viral interactionsVirologyJanuary 1, 2022Salomé Manska, Cyprian C. RossettoIE2 1:1000 (Millipore, MAB8140), UL112/113 1:750 (22), H1 1:50 (Santa Cruz, AE-4) H2A 1:200 (Abcam, ab18255), TOP2β 1:100 (Santa Cruz, H286), RBBP4 1:300 (Sigma, R3779), RNA POL II 1:500 (Abcam, ab5095), PARP

(12) SIRT1 coordinates with the CRL4B complex to regulate pancreatic cancer stem cells to promote tumorigenesisCell death and differentiationDecember 1, 2021Leng S, Huang W, Chen Y, Yang Y, Feng D, Liu W, Gao T, Ren Y, Huo M, Zhang J, et al.anti-OCT4 (ab19857), anti-SOX2 (ab59776), anti-c-Myc (ab32072), anti-NANOG (ab109250), and anti-H2A (ab18255) from Abcam; anti-SIRT3 (5490), anti-SIRT5 (8782), anti-SIRT6 (12486), anti-SIRT7 (5360), anti-H3K9ac

(13) The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscleNature communicationsOctober 14, 2021Rigillo G, Basile V, Belluti S, Ronzio M, Sauta E, Ciarrocchi A, Latella L, Saclier M, Molinari S, Vallarola A, et al.Santa Cruz Biotechnology), rabbit anti-phospho(S139)-H2AX (1:1000, #2577 Cell Signaling), rabbit anti-histone H2A (1:1000, Ab18255 Abcam), goat anti-Actin (I-19) (1:5000, sc-1616, Santa Cruz Biotechnology), mouse anti-Vinculin (V4504,

(14) Linking nuclear matrix–localized PIAS1 to chromatin SUMOylation via direct binding of histones H3 and H2A.ZJournal of Biological ChemistryOctober 1, 2021Zhaosu Chen et al.antibodies were used in this study: SUMO1 (ABclonal, A2130), histone H3 (Abcam, ab1791), histone H2A (Abcam, ab18255), histone H2B (Abcam, ab1790), histone H4 (Abcam, ab10158), histone H2A.Z (Abcam, ab4174), Lamin A/C

(15) UV-induced reduction in Polycomb repression promotes epidermal pigmentationDevelopmental cellSeptember 27, 2021Li MY, Flora P, Pu H, Bar C, Silva J, Cohen I, Galbo PM Jr, Liu H, Yu X, Jin J, et al.RRID: AB_476743" Rabbit anti-H3,Abcam,"Cat # ab1791, RRID: AB_30261" Rabbit anti-H2A,Abcam,"Cat # ab18255, RRID: AB_470265" Mouse anti-CD34,Thermo Fisher Scientific,"Cat # 14-0341-82, RRID: AB_467210" Rabbit

(16) H3K36me3 and PSIP1/LEDGF associate with several DNA repair proteins, suggesting their role in efficient DNA repair at actively transcribing lociWellcome Open ResearchSeptember 14, 2021Jayakumar Sundarraj, Gillian C.A. Taylor, Alex von Kriegsheim, Madapura M Pradeepaperformed by immunoblotting with MLL1 (Active Motif, 61295), γH2A.X (Millipore, 05-636), H2A (Abcam, ab18255) and H3K36me3 (Abcam, ab9050) antibodies. Comet assay Comet assays for WT and Psip1 knockout MEFs

(17) Tumor-derived neomorphic mutations in ASXL1 impairs the BAP1-ASXL1-FOXK1/K2 transcription networkProtein & CellJuly 1, 2021Yu-Kun Xia et al.(Abcam, catalog ab193203), anti-H3K27me3 (Abcam, catalog ab6002), anti-Histone H2A (Abcam, catalog ab18255), anti-Histone H3 (CST, catalog 9715), anti-TXNIP (Abcam, catalog ab188865), anti-HIF-1α (Abcam, catalog

(18) ER-directed TREX1 limits cGAS activation at micronucleiMolecular cellFebruary 18, 2021Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J.RRID:AB_627695 GFP,Invitrogen,Cat#A-11120; AB_221568 H3K9ac,Cell signaling technology,Cat#9649; RRID:AB_823528 Histone H2A,Abcam,Cat#ab18255; RRID:AB_470265 LaminB1,Abcam,Cat#ab16048; RRID:AB_443298 LSD1,Cell signaling technology,Cat#2184;

(19) Protective Effects of Sesamin on Cytoxan-Induced Spermatogenesis Dysfunction by Regulating RNF8-ubH2A/ubH2B Pathways in Male MiceFrontiers in pharmacologyJanuary 1, 2021Hai DM, Ren JW, Chi YN, Ye RJ, Liu N, Ma L, Lan XB, Wu J, Yu JQ, Yang JM.nonfat dry milk for 2 h and then incubated overnight at 4°C with the following antibodies: H2A (1:1,000, ab18255, Abcam, United Kingdom), H2B (1:10,000, ab1790, Abcam, United Kingdom), and RNF8 (1:800, 14112-1-AP,

(20) Chromosomal localization of Ewing sarcoma EWSR1/FLI1 protein promotes the induction of aneuploidyJournal of Biological ChemistryJanuary 1, 2021Hyewon Park, Haeyoung Kim, Victoria Hassebroek, Yoshiaki Azuma, Chad Slawson, Mizuki Azumawestern blot of the loading controls was treated with a 1:1000 dilution of rabbit anti-Histone H2A (Abcam, #ab18255) and a 1:2000 dilution of mouse anti-β-tubulin (Sigma-Aldrich, T4026) antibodies. All images of western blots

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Anti-Histone H2A antibody - ChIP Grade from Abcam

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Anti-Histone H2A antibody - ChIP Grade from Abcam

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